Domenico di Bartolo (c. 1400/1404 – 1445/1447) was an Italian painter of the Sienese School. He was born in Asciano. According to Vasari, he was a nephew of Taddeo di Bartolo. He was employed by Vecchietta in the masterpiece fresco The Care of the Sick in the Pellegrinaio (Pilgrim's Hall) of the Ospedale di Santa Maria della Scala in Siena. It portrays wealthy donors visiting the hospital to men washing the ill, and a fatty friar hearing confession. In 1434, he also painted a fresco panel of Emperor Sigismund Enthroned for the Siena Cathedral. Domenico died in Siena around 1445.