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Fibroblast responses to acetylcholine

Quail fibroblast responses to suberyldicholine (SDC). A Phase contrast morphology. B Control Ca++i signal. C Peak Ca signal. D Washing cells returns Ca level to control value (purple (100nM) to pink (over 1 microM). E cells after ionomycin to saturate dye. F After EGTA calibration. Credit: Biosciences Imaging Group,Southampton University/Wellcome Trust Medical Photo Library